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Bioinformatic Workflows

Exploratory gene expression analysis

Given the increased availability of data resources from large scale sequencing consortia, an efficient exploratory analysis could provide a tremendous research opportunity. This exploration can help generate novel hypothesis for experimentation, validate observations seen on smaller cohorts, and help improve fundamental understanding of genes and their roles in complex biological networks. In this page we will collect a list of public sequencing consortia where RNAseq data, raw and/or processed is available. Additionally, a set of analysis that can be performed on this datasets can be found below. If you want to perform a exploratory analysis using a public RNAseq database not stated below or want a specific analysis, please contact the i3S bioinformatic service.

Available databases

The Cancer Genome Atlas (TCGA) is a landmark cancer genomics program that molecularly characterized over 20000 primary cancer and matched normal samples spanning 33 cancer types.

The GTEx project has created a reference resource of gene expression levels from ‘normal’, non-diseased tissues. Currently, the project has analysed over 17000 samples from 54 different tissues from a total of 948 donors.

Available analysis

Principal component analysis

Heatmap analysis

Differential expression analysis

Pathway analysis

Predictive modeling